MALDI Sample Preparation: the Ultra Thin Layer Method
نویسندگان
چکیده
منابع مشابه
6.5 Evidence of Poor Maldi Sample Preparation
As described above, successful MALDI sample preparation involves creating inti mate contact between the matrix (which may consist of a few different molecules) and the analyte. The experiment requires each of the matrix roles to be completed to obtain a usable mass spectrum. To demonstrate the importance of the matrix roles, Figures 6.7 and 6.8 show two examples where a specific role of the ma...
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Ion abundances in the MALDI TOF mass spectra of the model peptides (bradykinin, alpha-melanocyte stimulating hormone, and melittin) change significantly as water is added to the solution used for dried droplet sample preparation. Changes in the surface tension of the solution can be invoked to explain some of the observed effects on the mass spectra. For example, at low surface tensions, the so...
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Ultrathin-layer chromatography (UTLC) is a recently developed analytical method intended for compact, rapid separations of nanolitre analyte volumes. Optimizing this method's performance requires new measurement techniques compatible with the millimetre length scales and rapid separation dynamics observed in UTLC. We have designed, implemented and characterized a measurement system which record...
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ژورنال
عنوان ژورنال: Journal of Visualized Experiments
سال: 2007
ISSN: 1940-087X
DOI: 10.3791/192